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Antibiofilm Activity of ZnO/Zeolite Nanocomposite (ZnO/ZeoNC) Against Klebsiella pneumoniae and Biocompatibility in an Animal Model

Author(s):

Alireza Partoazar, Fatemeh Rahmani Bideskan, Nasrin Takzaree and Mohammad Mehdi Soltan Dallal*   Pages 1 - 8 ( 8 )

Abstract:


Background: Infectious diseases, whether intracellular or extracellular infections, biofilm-mediated, or medical device- associated have always been a global problem in public health causing millions of deaths each year. The aim of this study was to evaluate the antibiofilm activity of ZnO/ZeoNC against K. pneumoniae along with biocompatibility of nanocomposite in vivo model.

Objective: The formation of biofilm by K. pneumoniae in the catheter-associated urinary tract causes a nosocomial infection. In this regard, antimicrobial nanomaterials have emerged as potent effective agents against biofilm formation. Nevertheless, nanoparticles have already been a challenge with possible side effects such as inflammation. The ZnO/ZeoNC may exhibit anti-biofilm property with minimal adverse effects.

Methods: The biofilm formation of K. pneumoniae strains was exposed to ZnO/ZeoNC and then SEM imaging was performed for morphological investigation of bacteria in biofilm state. The quantity of mice tissue response to ZnO/ZeoNC embedded polyethylene tube also analyzed on the tissue of mice during the 30-day experiment.

Results: The results of this study showed that ZnO/ZeoNC has the significant antibiofilm activity against K. pneumoniae strains in its sublethal doses. The ZnO/ZeoNC also caused deformation on K. pneumoniae biofilm. In addition, ZnO/ZeoNC also reduced inflammatory response in cell tissue of rats subjected to polyethylene tube.

Conclusion: ZnO/ZeoNC can be used potentially against the infections due to K. pneumonia biofilm without irritability on the biotic surface such as urinary tract.

Keywords:

Anti-biofilm, nosocomial infection, nanoparticle, inflammation, urinary tract.

Affiliation:

Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran



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